Composite

Part:BBa_K1212012:Design

Designed by: Amy Soon   Group: iGEM13_UC_Davis   (2013-09-16)


pBAD+Riboswitch2+TAL8


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal PstI site found at 180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 203
    Illegal BamHI site found at 2597
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 180
    Illegal NgoMIV site found at 221
    Illegal NgoMIV site found at 2423
    Illegal AgeI site found at 2604
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There cannot be a scar between the end of the riboswitch and the start codon of the TAL repressor.


Source

The pBAD is a part from the registry. The riboswitch is from the Gallivan Lab's paper A flow cytometry-based screen for synthetic riboswitches. by Lynch et al. in Nucleic Acids Res. The TAL repressor is from the Segal Lab at the UC Davis Genome Center. See their paper Quantitative Analysis of TALE-DNA Interactions Suggests Polarity Effects. by Meckler et al. in Nucleic Acids Res.

References

S. A. Lynch and J. P. Gallivan, "A flow cytometry-based screen for synthetic riboswitches," Nucleic Acids Research, vol. 37, pp. 184-192, Jan 2009.

J. F. Meckler, M. S. Bhakta, M. S. Kim, R. Ovadia, C. H. Habrian, A. Zykovich, et al., "Quantitative analysis of TALE-DNA interactions suggests polarity effects," Nucleic Acids Res, vol. 41, pp. 4118-28, Apr 2013.